Inactivation of Lambda Phage Infectivity and Lambda Deoxyribonucleic Acid Transfection by N -Methyl-Isatin β-Thiosemicarbazone–Copper Complexes
نویسندگان
چکیده
منابع مشابه
Infectivity of lambda heteroduplex deoxyribonucleic acid molecules.
Heteroduplex deoxyribonucleic acid (DNA) molecules were formed in vitro by denaturing and renaturing a mixture of DNAs from a variety of lambda strains. The infectivity of these heteroduplexes was studied by using host-controlled modification and restriction to prevent infection by contaminating parental homoduplexes. Either strand was able to protect against degradation by restriction nuclease...
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The terminase motors of bacteriophages have been shown to be among the strongest active machines in the biomolecular world, being able to package several tens of kilobase pairs of viral genome into a capsid within minutes. Yet these motors are hindered at the end of the packaging process by the progressive build-up of a force resisting packaging associated with already packaged DNA. In this exp...
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Large amounts of a heat-inducible phage lambda mutant (lambdaCI857) may be obtained under standardized conditions. The phage is harvested by simple polyethylene glycol (C 6000) precipitation and purified by CsCl density gradient banding. Deoxyribonucleic acid (DNA) is extracted by cold phenol and purified by sucrose density gradient sedimentation to yield a homogenous population of unbroken lam...
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1 Faculty of Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8512, Japan 2 Department of Automatic Control and Systems Engineering, Technical University of Ilmenau, 98684 Ilmenau, Germany 3 Department of Information Science, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan 4 Human Genome Center, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokaneda...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1976
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.9.1.160